Figure 6.

MAGI2-AS3 inhibits the LSCs self-renewal by promoting LRIG1 DNA demethylation via TET2 recruitment. A: protein expression of TET2 is determined by western blot analysis; B: mRNA expression of LRIG1 is tested by RT-qPCR; C: protein expression LRIG1 is determined by western blot analysis D: LSCs sphere formation using sphere formation assay (× 200); E: LSCs colony formation using colony formation assay; * p < 0.05 vs. the oe-MAGI2-AS3 + sh-NC group; data were expressed as mean ± standard error and analysed using unpaired t-test. The experiment was repeated three times independently.