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RT-qPCR primer sequences for fractionation of nuclear and cytoplasmic RNA.
| Gene | Primer Sequence |
|---|---|
| 45S rRNA | Forward: 5ʹ-CCGGCGCAAGACGGACCAGA-3’ |
| Reverse: 5ʹ-CATTCTTGGCAAATGCTTTCG-3’ | |
| 12S rRNA | Forward: 5ʹ-ATATCTGAACACACAATAGCTAAGAC-3’ |
| Reverse: 5ʹ-TAGAGGGATATGAAGCACCG-3’ |
RT-qPCR, quantitative real-time polymerase chain reaction.
