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Primer sequences for methylation-specific PCR.
| Gene | Primer sequence |
|---|---|
| LRIG1-M | Forward: 5ʹ-TTGTTTAATTTTTTTGATAATGTGC-3’ |
| Reverse: 5ʹ-AACATATAACTACCCTCTAAACGTT-3’ | |
| LRIG1-U | Forward: 5ʹ-GTTTAATTTTTTTGATAATGTGTGT-3’ |
| Reverse: 5ʹ-AACATATAACTACCCTCTAAACATT-3’ |
M, methylated-specific primers; U, unmethylated-specific primers; LRIG1, leucine-rich repeats and Ig-like domains 1.
