Figure 4.
Transfected RNAs are secreted in EVs and delivered to acceptor cells in a donor cell concentration-dependent manner. (A) Quantitation of tRNAGly 5ʹ halves (red; used for transfection) and miR-21-5p (green; not transfected) in EVs, as a function of the concentration of RNA used for transfection. (B) Relative quantitation of tRNAGly 5ʹ halves (normalized to miR-21-5p levels) in acceptor cells, as a function of the concentration of RNA used for donor cell transfection. (C) Correlation between RNA levels in EVs and in acceptor cells. For all these experiments, a fixed donor to acceptor cell ratio of 4:1 was used. (D) Same as (B) but varying the donor to acceptor cell ratio at a fixed RNA concentration of 100 nM (red). EVs were also purified from not transfected (black) or – L2K cells (blue).