Table 3.
Significant fold changes in the expression of rnjA, rnjB, rnc, rny, cshA, cshB, cshC, in E. faecalis WT strain exposed to 30 min of stress or during mice infection and compared to exponential growth cells at 37°C in BHI.
Stress conditions | rnjA (J1) | rnjB (J2) | rny (Y) | rnc (III) | cshA | cshB | cshC |
---|---|---|---|---|---|---|---|
pH5.7, 37°C | −14.2 | −21.0 | 9.2 | NS | 2.5 | NS | NS |
0.06% BS, 37°C | 3.5 | 5.2 | 5.3 | 6.3 | 4.2 | NS | NS |
1 mM H2O2, 37°C | −29.8 | −28.3 | 10.0 | 3.4 | 7.3 | −2.2 | NS |
BHI, 15°C | 5.7 | 2.6 | 5.9 | 4.2 | 12.2 | 9.3 | 5.3 |
mice peritonitis | 16.2 | 12.5 | 9.1 | 2.5 | 6.3 | NS | NS |
The level of expression was measured by RT-qPCR and compared to the expression of the housekeeping gene gyrA. NS: non-significant, in bold: induced expression.