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tRFs analyses. (A) tRFs-piRNA expression in each sample in total reads percentage. (B) Schematic view of two tRFs from tRNAs split: 5ʹ and 3ʹ (for 3ʹ fragments the CCA-tail is not considered. (C) Read length distribution for each tRFs (5ʹ and 3ʹ) in PGC and SC. (D) The 15 most expressed tRFs. The tRNA-derived piRNAs are mostly 5ʹ tRFs and have a read length about 28–33 nt. The 95% of these piRNAs derived from most expressed 8 ones.
