Figure 3.

Structural insights into cap substitutions and other cap-binding proteins. Panel A. NMR based model of eIF4E/VPg complex highlighting the competition with the m⁷G cap in the cap-binding pocket (red arrow). Panel B. eIF4E2 bound to TRS, using a similar surface used by eIF4E to bind eIF4G or 4E-BP1. The crystal structure was not solved in the presence of a cap analogue, but the cap-binding pocket is shown with the red arrow. Panels C and D. Structures of factors identified demonstrating the similar means by which they interact with the m⁷G cap. This same pocket is use to bind TMG cap for CBC. Panel E. eIF3d cap-binding domain modelled with the cap analogue placed according to its position in DXO. PDB codes are as follow: 5XLN (B); 1H2U (C); 3D45 (D); 5K4B (E).