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. 2020 Mar 6;17(7):918–929. doi: 10.1080/15476286.2020.1735210

Figure 2.

Figure 2.

Biogenesis and expression analyses of multi-miRshRNA in transgenic Ae. aegypti. (A) Schematic diagram showing processing multi-miRshRNA of RNAiDENV to miRNA by RNAi pathway. (B) Relative expression levels of DENV target sequences in TM with respect to the reference, Ae. aegypti microRNA, mir-1174 at 1 dpi, (C) Gel image of miRshRNAs and mir-1174 expressions at 1 dpi in WM and TM. Three replicates were performed in analysing relative expression levels of DENV target sequences at 1 dpi. Bars display mean ± SEM (Standard Error Mean) (TM: transgenic mosquito, WM: wild mosquito, miRshRNA: microRNA based shRNA, multi-miRshRNA; multiple miRshRNA, pri-miRNA: primary miRNA, pre-miRNA: precursor miRNA, siRNA: small interfering RNA, Dicer and Drosha are enzymes involved in RNA interference mechanism, RISC: RNA-induced silencing complex, RNAiDENV: Designed gene with AeCPA/multi-miRshRNA/svA, NRTC: No reverse transcriptase control, NTC: No template control).