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. 2020 Mar 1;17(8):1116–1124. doi: 10.1080/15476286.2020.1732702

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Figure 4. — Endogenous mature tRNAs can be also purified. Mature tRNA-Ala-AGC (tRNA-Ala) and tRNA-Gly-GCC (tRNA-Gly) were pulled down using gel-purified mature tRNA fraction. (A) SYBR Gold staining and (B) Northern blotting for tRNA-Ala-AGC, tRNA-Gly-GCC, tRNA-His-GTG and tRNA-iMet-CAT. (C) INB for 7-methylguanosine (m7G) and pseudouridine modifications. In pulldown (PD) fraction, bands indicated by red arrowheads show the purified specific tRNAs. m7G and pseudouridine modifications are indicated as red circle and blue circle, respectively. Sup: supernatant fraction, PD: pulldown fraction.