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Amplification and sequencing of oxidative stress-induced cP-RNAs.
(A) Total RNAs extracted from SA-treated HeLa or U2OS cells were subjected to northern blots for the 5′-halves of cyto tRNAHisGUG. (B) Gel-purified 20–45-nt RNAs were subjected to cP-RNA-seq, which amplified 140–160-bp cDNA products (5′-adapter, 55 bp; 3′-adapter, 63 bp; and thereby estimated inserted sequences, 22–42 bp).The cDNAs in the region designated with a line were purified and subjected to Illumina sequencing.
