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. 2020 Jun 30;17(10):1391–1401. doi: 10.1080/15476286.2020.1771519

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Figure 3. — Knockdown of NR_003529 in HCAEC, HUVECs and EA.hy926 cells reduces monocyte adhesion and transmigration. (A) Expression of the NR_003529 transcript of ANRIL in HCAECs, HUVECs, and EA.hy926 cells transiently transfected with NR_003529-specific siRNA (siNR) vs. negative control siRNA (siNC) as quantified by qRT-PCR. (B) Expression of the DQ485454 transcript of ANRIL in HCAECs, HUVECs, and EA.hy926 cells transiently transfected with NR_003529-specific siRNA (siNR) vs. negative control siRNA (siNC) as quantified by qRT-PCR. (C) Relative adhesion of monocytes to HCAECs, HUVECs, and EA.hy926 cells transiently transfected with NR_003529-specific siRNA (siNR) vs. negative control siRNA (siNC). (D) Relative transmigration of monocytes across a layer of HCAECs, HUVECs, and EA.hy926 cells transiently transfected with NR_003529-specific siRNA (siNR) vs. negative control siRNA (siNC). *p < 0.05 and **p < 0.01 vs. vector, N = 5.