Figure 2.

Effect of DSS40 on permeability of polar – and lipophilic probes. Explants from pig colon were cultured for 1 h in the absence/presence of 2% DSS40, and in the presence of both OGD10 and TRD70 or in the presence of FM, as described in Methods. Representative images of cryosections of the labeling profiles of each of the polar probes are shown together with a merged image. L; lumen, C; crypt, LP; lamina propria. Arrows indicate a stripy labeling of the lateral sides of the colonocytes in the merged image of the left panel and goblet cells in the images with FM. Bars: 50 µm.