Figure 1.

(A) TEER value progressions of submerged and airlift cultivation in final EpiLife media (E3) shown as Ω x cm² (mean ± SEM, n = 3–12 inserts from four independent experiments). Statistical analysis was performed with two-way ANOVA following post hoc Tukey's test, with α = 0.05, ***p < 0.001. (B) Permeability coefficient values [µm/min] of carboxyfluorescein on the last day of experiments after cultivation in final EpiLife media (E3) (mean ± SD, n = 8–9 inserts from three independent experiments). Statistical analysis was performed with Student’s t-test (airlift vs submerged), α = 0.05, ***p < 0.001. (C) TEER value progressions of submerged and airlift cultivation in DMEM media shown as Ω x cm² over time (mean ± SEM, n = 3–18 inserts from six independent experiments). Statistical analysis was performed with two-way ANOVA following Holm–Sidak as post hoc test, with α = 0.05, *p <0.05, **p < 0.01 ***p < 0.001. (D) Permeability coefficient values [µm/min] of carboxyfluorescein on the last day of experiments after cultivation in DMEM media under submerged and airlift condition (mean ± SD, n = 15 inserts from five independent experiments).