Figure 2.

Cultivation of TR146 cells on inserts in DMEM with supplements monitored with TEER and permeability of carboxyfluorescein (A) Progression of TEER values versus time as x-fold. Ω x cm² values of submerged cultivation in DMEM media (●) was used as reference for airlift cultivation in DMEM media (○), supplemented with 10 (▼), 100 (∆) or 1000 nM (□) HC. Statistical analysis was performed as two-way ANOVA with Holm–Sidak test (mean ± SD, n = 9 inserts from three independent experiments, α = 0.05, *p < 0.05, **p < 0.01, ***p < 0.001). (B) Corresponding permeability coefficients of carboxyfluorescein on the last day of experiments (mean ± SD, n = 9 inserts from three independent experiments, one-way ANOVA, Tukey test) to 2A. (C) Comparison of submerged cultivation in DMEM (●) to airlift in DMEM (○), supplemented with 1% HKGS (▼) or HKGS/KGF/A2P (∆) (mean ± SD, n = 9–12 inserts from three independent experiments, two-way ANOVA with Holm–Sidak test). (D) Permeability values of carboxyfluorescein on the last day of experiments corresponding to 2C (mean ± SD, n = 8 inserts from two independent experiments, one-way ANOVA, Dunn´s test).