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. 2020 Apr 21;8(2):1748459. doi: 10.1080/21688370.2020.1748459

Figure 5.

Figure 5.

Expression values of (A) cytokeratins, cornification, and EMT (epithelial–mesenchymal transition) markers (B) mucins and (C) aquaporins of the High-Throughput qPCR Chip shown as ∆Ct values, referred to PPIA (peptidylprolyl isomerase A) as endogenous control. TR146 samples [Samples 1–39, 1–3: cultivated in EpiLife media (E3), submerged; 4–6: EpiLife (E3), airlift; 7–11: DMEM media, submerged; 12–16: DMEM media, airlift; 17–19: DMEM media supplemented with 1% HKGS, airlift; 20–22: DMEM media supplemented with HKGS/KGF/A2P, airlift; 23–25: DMEM media supplemented with 10 nM HC, airlift; 26–28: DMEM media supplemented with 100 nM HC, airlift: 29–31: DMEM media supplemented with 1000 nM HC, airlift: 32–33: DMEM media w/o serum, airlift; 34–36: DMEM media w/o serum supplemented with 1% HKGS, airlift: 36–39: DMEM media w/o serum supplemented with HKGS/KGF/A2P, airlift] plotted against 8 biopsy samples (B1-8) of the oral mucosa.