Table 1.
Overview of targets expressed by both the TR146 cell line and the biopsy samples upon PCR or real-time PCR showing nine different categories of markers. Abbreviations are explained below the table (all biopsy samples additionally expressed tight junction protein CLDN5, 5 out of 8 samples expressed MUC19).
| Endogenous controls | 18sRNA | PPIA | GAPDH | ß-actin | B2M |
|---|---|---|---|---|---|
| Claudins | CLDN1 | CLDN2 | CLDN3 | CLDN4 | CLDN6 |
| CLDN7 | CLDN8 | CLDN9 | CLDN10 tva | CLDN10 tvb | |
| CLDN11 | CLDN12 tv1 | CLDN12 tv2 | CLDN12 tv3 | CLDN14 | |
| CLDN15 | CLDN16 | CLDN17 | CLDN18 tv1b | CLDN18 tv2b | |
| CLDN20 | CLDN22 | CLDN24 | CLDN 25 | ||
| Other Tight Junctions | ZO-1 | ZO-2 | ZO-3 | JAM-1 | JAM-3 |
| occludin | tricellulin | ||||
| Transporter proteins | P-gp (ABCB1) | MRP1 (ABCC1) | MRP2 (ABCC2) | MRP3 (ABCC3) | MRP4 (ABCC4) |
| MRP5 (ABCC5) | BCRP (ABCG2) | CAT1 (SLC7A1) | GLUT1 (SLC2A1) | LAT1 (SLC5A7) | |
| MCT1 (SLC16A1) | MCT8 (SLC16A2) | ||||
| Receptors | TfR | InR | LRP1 | LRP8 | |
| EMT | E-cadherin | ß-catenin | vimentin | VEFG-A | fibronectin |
| Cornification | filaggrin | involucrin | loricrin | ||
| Cytokeratins | CK1 | CK4 | CK5 | CK8 | CK10 |
| CK13 tv1 | CK13 tv2 | CK14 | CK16 | CK18 | |
| CK19 | |||||
| Aquaporins | AQP1 | AQP3 | AQP4 | AQP7 | AQP9 |
| AQP10 | AQP11 | ||||
| Mucins | MUC1A | MUC1B | MUC1 tv9 | MUC2 | MUC3A |
| MUC4 | MUC7 | MUC13 | MUC15 tv1,3 | MUC15 tv2 | |
| MUC16 | MUC18 | MUC19 | MUC20 | MUC21 |
ABC … ATP-binding cassette, AQP … Aquaporin, B2M … β2-Microglobulin, BCRP … Breast Cancer Resistance Protein, CK … Cytokeratin, CLDN … Claudin, EMT … Epithelial–mesenchymal transition, GAPDH … Glyceraldehyde-3-phosphate dehydrogenase, InR … Insulin receptor, JAM … Junctional Adhesion Molecule, LRP …Low density lipoprotein (LDL) receptor-related protein, MCT … Monocarboxylate-transporter, MRP … Multidrug Resistance-Associated Protein, MUC … Mucin, P-gp … P-glycoprotein, PPIA … Peptidylprolyl isomerase A, SLC … Solute carrier, TfR … Transferrin receptor, tv … transcript variant, VEFG … Vascular endothelial growth factor, ZO … Zonula occludens.