Table 2.
Comparison of highly expressed targets in human oral biopsy samples (n = 8) versus TR146 cells cultivated under optimized conditions (DMEM media, supplemented with HKGS, airlift, n = 3, final TEER of 115.39 ± 38.48 Ω x cm2). Ct values were obtained with the 96.96 High-Throughput qPCR-Chip, referred to PPIA (peptidylprolyl isomerase A) as housekeeping gene, normalized to the expression values of biopsy samples and listed as x-fold values (mean ± SEM). Statistical analysis was performed using the Student´s t-test with α = 0.05, *p <0.05, **p < 0.01, ***p < 0.001.
| Target groups | Targets | TR146 | Biopsies | p-value |
|---|---|---|---|---|
| Tight Junctions | CLDN1 | 1.69 ± 0.23 | 1 ± 0.42 | |
| CLDN4 | 0.81 ± 0.08 | 1 ± 0.22 | ||
| CLDN7 | 0.76 ± 0.07 | 1 ± 0.40 | ||
| JAM1 | 1.61 ± 0.22 | 1 ± 0.16 | ||
| Transporters | LAT1 | 4.57 ± 0.38 | 1 ± 0.21 | ** |
| Cytokeratin | CK4 | 0.08 ± 0.05 | 1 ± 0.59 | |
| CK5 | 0.47 ± 0.21 | 1 ± 0.18 | ||
| CK10 | 2.58 ± 1.46 | 1 ± 0.36 | ||
| CK13 | 0.11 ± 0.05 | 1 ± 0.20 | ** | |
| CK14 | 0.52 ± 0.14 | 1 ± 0.20 | ||
| CK18 | 1.42 ± 0.77 | 1 ± 0.42 | ||
| Cornification | Involucrin | 3.99 ± 1.75 | 1 ± 0.23 | |
| Aquaporins | AQP3 | 0.63 ± 0.26 | 1 ± 0.17 | |
| Mucins | MUC21 | 0.003 ± 0.002 | 1 ± 0.56 |