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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: Macromol Biosci. 2020 Mar 20;20(5):e1900445. doi: 10.1002/mabi.201900445

Figure 1.

Figure 1.

Characterizations of Fab’-MORF1 and P-(MORF2)X with size exclusion chromatography AKTA FPLC. Superdex 200 10/300 GL column (GE Healthcare) eluted with phosphate buffered saline (PBS, pH 7.2) at flow rate of 0.4 ml min−1 was used for Fab’-MORF1 characterization. Superose 6 HR10/30 column with sodium acetate buffer containing 30% acetonitrile (pH6.5) at flow rate of 0.4 ml min−1 was used for P-(MORF2)X characterization. A. Full antibody was digested to F(ab’)2 (green) followed by reduction to obtain Fab’(red). Target peak shifted back after Fab’ conjugated with MORF1 (blue); B. Polymer peak shifted significantly after MORF2 conjugation (green).