(A) Sperm from wild-type and Tbc1d21-/- mice stained with SEPT7 antibody (green), Mito-tracker (red), and DAPI (blue). Figures from left to right indicate the bright field (Bright field), enlarged square from bright field, immune-staining figure (DAPI, Mito-tracker, and SEPT7), and enlarged square from immune-staining figure. Arrows point out the where annulus sits (SEPT7 signals, green). Figure a. for wild-type mice, and Figures b.-d. from Tbc1d21-/- mice. (B) Sperm from wild-type (a.) and Tbc1d21-/- (b. and c.) mice stained with Mito-tracker (red), and Basigin antibody (green). Figures from left to right indicate bright field, staining with the Mito-tracker (red), immune-staining with Basigin antibody (green) and merged figures. The length of the midpiece is indicated between the white arrows.