Fig 1. DomI expression and purification from the culture medium.

(A) Reducing SDS-PAGE of the culture supernatant. Samples were taken every 24 h during the 96 h of methanol induction. After centrifugation to remove cells, supernatants were diluted in 4 x sample buffer and 15 μL were loaded. Empty lanes were cropped. (B) Picture of the sample obtained after culture centrifugation, protein precipitation with ammonium sulfate, pellet resuspension and dialysis.