Fig 3. DomI oligomeric state.

Purified DomI (100 μL, 90 μg/mL) was loaded on a Superdex 75 10/300 column previously equilibrated with Tris (50 mM, pH 8.0, 100 mM NaCl) at 0.8 mL/min and absorbance at 280 nm was registered. Arrows indicate the elution volume of the proteins used in the calibration.