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Purified DomI was reduced under denaturing conditions, alkylated with iodoacetic acid and trypsinized. (A) MALDI-TOF MS of DomI tryptic fragments. The peak with m/z 2491.23 (in bold) corresponds to the Cys34-containing peptide, carboxymethylated (CM, + 58). Inset: m/z 2491.23 peptide isotopic distribution. (B) MALDI-TOF MS/MS of the parent ion at m/z 2491.23 showing the identified b and y fragments.
