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. 2020 Oct 12;15(10):e0240580. doi: 10.1371/journal.pone.0240580

Fig 5. Selective reduction of Cys34 and MALDI-TOF MS analysis.

Fig 5

Purified DomI was incubated with 2-mercaptoethanol and treated with iodoacetamide. Then, DTT was added to the mixture followed by iodoacetic acid treatment and trypsinization. (A) MALDI-TOF MS of DomI tryptic fragments. Carboxymethylated (CM) and carbamidomethylated (CAM) Cys-containing peptides are shown. Inset: m/z 2490.33 peptide isotopic distribution. (B) MALDI-TOF MS/MS of the parent ion at m/z 2490.33 showing the b and y fragments identified.