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. 2020 Oct 8;12:9813–9824. doi: 10.2147/CMAR.S264358

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© 2020 Zhang et al.

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Validation of enhanced MUC1-CAR-T cells after long-term stimulation in vitro. (A) The proliferation and accumulation of traditional and enhanced MUC1-CAR-T cells, and the number of tumor cells during continuous co-culture. (B) The number of CAR-T cells during the 5-week incubation, counted every 7 days. (C) After 5 weeks of incubation, T cells were collected and the level of TNF-α and IFN-γ, secreted by CAR-T cells, was determined by flow cytometry. (D) After 5 weeks of incubation, T cells were collected and flow cytometry was applied to determine the expression of cell depletion biomarkers on the surface of T cells, including PD-1, TIM-3, and LAG-3. (E) The expression of PD-1, TIM-3, and LAG-3 (n = 3, bar value represents the degree of dispersion, Two-way ANOVA, ns is no significant difference, *P<0.05).