Figure 4.

Recombinant SmNPP5 prevents NAD induced T cell death (NICD)in vitro. Panels athough dare representative flow cytometry dot plots of purified T cells following exposure to NAD (panel b) or not (Control, panel a) or to NAD that had been incubated with1 µg rSmNPP5 for 1 hour (panel c) or for 5 hours (panel d). The “Key” depicted in Figure 2 (lower left) applies here too; the percentages of total apoptotic cells are bounded by blue boxes. Panel erepresents composite data from replicate experiments, showing the mean percent total apoptotic T cells (± SEM) following the treatments indicated. *p<0.05 1 h v 5 h, ****p<0.0001+ NAD at time 0 v all other groups, One-way ANOVA, n≥4 in each case.