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. 2020 Aug 20;11(1):1059–1074. doi: 10.1080/21505594.2020.1806664

Figure 1.

Figure 1.

The factor H binding ability of M. hyopneumoniae strain 168 and its influence on M. hyopneumoniae survival in swine sera.

(a) The factor H binding ability of M. hyopneumoniae strain 168 incubated with rabbit anti-factor H polyclonal antibodies and horseradish peroxidase (HRP)-conjugated goat anti-rabbit polyclonal antibodies. Background values were obtained for the control (no factor H or wells coated with M. hyopneumoniae culture medium). The OD450 was used for the statistical analysis. Each assay was performed in triplicate wells, and repeated independently three times(b) The survival of M. hyopneumoniae cells in the presence of normal swine sera (used as a complement pool) as determined by assaying for CCU (color change unit) after incubating with an equal volume of factor H or PBS. Three samples were assayed in each experiment.Differences were analyzed using the Student’s t- test. A p value ≤ 0.05 was considered highly significant (**). Mhp is short for M. hyopneumoniae.