Table 1.
Strains used in this study.
| Strain | Description | Source |
|---|---|---|
| E. coli PCN033 | Wild type ExPEC strain, highly virulent, isolated from pig brain | [27] |
| E. coli DH5α | Cloning host strain | Vazyme Biotech Co., Ltd. |
| E. coli DH5α λpir | Cloning host strain | [33] |
| E. coli χ7213 | Diaminopimelic acid autotrophic strain used in transconjugation. | [31] |
| Δtat | As E. coli PCN033, tatABC deleted. | This work |
| Δtat-Cm | As E. coli PCN033, tatABC replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔamiA | As E. coli PCN033, amiA replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔamiA2 | As E. coli PCN033, in-frame deletion of amiA. | This work |
| ΔamiC | As E. coli PCN033, amiC replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔamiAΔamiC | As ΔamiA2, amiC replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔsufI | As E. coli PCN033, sufI replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔmoaA | As E. coli PCN033, moaA replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔcueO | As E. coli PCN033, cueO replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔyahJ | As E. coli PCN033, yahJ replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔwcaM | As E. coli PCN033, wcaM replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔmodD | As E. coli PCN033, modD replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔfhuD | As E. coli PCN033, fhuD replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔycbK | As E. coli PCN033, ycbK replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔefeOB | As E. coli PCN033, efeOB replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔfdnG | As E. coli PCN033, fdnG replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔfdoG | As E. coli PCN033, fdoG replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔhyaA | As E. coli PCN033, hyaA coding sequence replaced with chloramphenicol resistance gene coding sequence. | This work |
| ΔnapG | As E. coli PCN033, napG replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔhybAO | As E. coli PCN033, hybAO replaced with chloramphenicol resistance cassette. CmR. | This work |
| ΔnrfC | As E. coli PCN033, nrfC coding sequence replaced with chloramphenicol resistance gene coding sequence. | This work |
| ΔyagT | As E. coli PCN033, yagT coding sequence replaced with chloramphenicol resistance gene coding sequence. | This work |
| ΔydhX | As E. coli PCN033, ydhX coding sequence replaced with chloramphenicol resistance gene coding sequence. | This work |
| CΔtat | ΔtatABC transformed with pHSG396-tatABC | This work |
| CΔsufI | ΔsufI transformed with pHSG396Apra-sufI | This work |