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. 2020 Aug 10;11(1):980–994. doi: 10.1080/21505594.2020.1797352

Figure 4.

Figure 4.

Ssads-mediated AR signaling facilitates S. suis translocation across the BBB in vitro. S. suis strains were added to confluent HCMEC/D3 monolayers at an MOI of 100. (a) The effects of selective AR antagonists DPCPX (100 nM; specific for A1 AR), ZM 241385 (1 µM; specific for A2A AR), and PSB 603 (1 µM; specific for A2B AR) on bacterial translocation across HCMEC/D3 monolayers at 1 h post-infection were determined. (b) The cellular cAMP content of HCMEC/D3 cells was measured 1 h post-infection with S. suis cells. (c) The effects of Rp-cAMPS (200 µM) – a competitive antagonist of cAMP-induced activation of PKA – on bacterial translocation across HCMEC/D3 monolayers at 1 h post-infection were determined. (d) The effects of the selective A1 AR agonist CCPA (1 µM) and adenosine (10 µM) on bacterial translocation across HCMEC/D3 monolayers at 1 h post-infection were determined. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, two-tailed Student’s t test.