Figure 5.

A1 AR activation promotes S. suis BBB penetration. (a) The translocation of S. suis across monolayers of HCMEC/D3 A1 AR-KO cells or HCMEC/D3 unedited cells was measured at 1 h post-infection. Data are expressed as means ± SEM. (b,c) Six-week-old female C57BL/6 mice were infected i.p. with approximately 5 × 10⁶ CFU of S. suis strains. Bacterial CFU in the blood and brain were measured from animals sacrificed at 72 h post-infection. Horizontal lines and error bars denote the median and IQR, respectively. (b) The effects of CCPA (0.37 mg/kg BW) i.v. at 2 h post-infection on the bacterial loads in the brain and blood were determined (n = 11 mice per group). (c) The effects of administration of DPCPX (1 mg/kg BW) concomitantly with bacterial suspension by the i.p. route on the bacterial loads in the brain and blood were determined (n = 8 mice per group). (d) Comparison of bacterial CFU recovered from the blood and recovered from brains of unedited and A1 AR-KO mice 72 h after i.p. challenge with 5 × 10⁶ CFU of S. suis WT strain were performed (n = 8 mice per group). Horizontal lines and error bars denote the median and IQR, respectively. *P < 0.05, **P < 0.01, ***P < 0.001, Mann-Whitney U test.