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Co-infection simulation experiments with three pathogens. A-D: amplification curves (X-axis: Cycle, Y-axis: Fluorescence) of PDCoV + PToV + SADS-CoV, PEDV + PToV + SADS-CoV, PEDV + PDCoV + SADS-CoV, PEDV + PDCoV + PToV with concentration of 1 × 103 copies/μL; E-H: amplification curves of PDCoV + PToV + SADS-CoV, PEDV + PToV + SADS-CoV, PEDV + PDCoV + SADS-CoV, PEDV + PDCoV + PToV with concentrations of 1 × 102 copies/μL. Two replicates were set per reaction.
