Table 2.

Cq values of PEDV, PDCoV, PToV, and SADS-CoV detected by this multiplex real-time PCR assay with different probe and primer concentrations.

PEDV					PDCoV
Probe concentration (nM)	Primer concentration (nM)				Probe concentration (nM)	Primer concentration (nM)
	1200	1600	2000	2400		1200	1600	2000	2400
200	31.14	29.75	29.46	29.58	200	30.97	29.07	28.54	28.29
400	30.50	29.49	28.47	29.40	400	30.20	28.60	27.36	28.09
600	29.78	29.19	29.04	29.02	600	29.73	28.31	28.01	27.92
800	29.43	29.18	28.91	28.91	800	29.14	28.21	27.90	27.93
1000	29.55	29.08	29.11	28.84	1000	28.42	27.94	28.13	27.83
PToV					SADS-CoV
Probe concentration (nM)	Primer concentration (nM)				Probe concentration (nM)	Primer concentration (nM)
	1200	1600	2000	2400		1200	1600	2000	2400
200	31.63	29.35	29.48	29.24	200	29.82	29.10	29.08	28.99
400	30.87	29.57	28.04	28.77	400	30.56	29.51	29.26	29.35
600	30.17	29.05	28.81	28.72	600	31.47	29.77	29.68	29.51
800	29.49	28.73	28.65	28.40	800	31.66	30.14	29.42	29.59
1000	28.57	28.40	28.17	28.05	1000	33.66	30.58	30.21	29.96