Table 3.

Sensitivity of the multiplex real-time PCR assay.

Pathogens	Concentration	Total	Positive	Detection rate	95% detection rate
PEDV	1000 copies/μL	23	23	100.0%	> 95%
	100 copies/μL	23	23	100.0%	> 95%
	10 copies/μL	23	20	87.0%	< 95%
PDCoV	1000 copies/μL	23	23	100.0%	> 95%
	100 copies/μL	23	23	100.0%	> 95%
	10 copies/μL	23	14	60.9%	< 95%
PToV	1000 copies/μL	23	23	100.0%	> 95%
	100 copies/μL	23	23	100.0%	> 95%
	10 copies/μL	23	17	73.9%	< 95%
SADS-CoV	1000 copies/μL	23	23	100.0%	> 95%
	100 copies/μL	23	23	100.0%	> 95%
	10 copies/μL	23	12	52.2%	< 95%

The cutoff line of positivity is automatically decided by Roche LightCycler® 96 Instrument.