Figure 8.
Changes in hemocyte counts in SAMTB lux infected larvae.
Total number of circulating hemocytes of naïve, PBS-T injected and SAMTB lux infected (2x107 CFU) larvae were determined at 2, 4, 24 and 48 h post-injection. Five larvae were bled (approximately 60 µl of hemolymph/larva) and diluted in PBS containing 0.37% of mercaptoethanol. Hemocytes were stained 1:1 with trypan blue (0.4%) and counted in a hemocytometer. Data are pooled from three independent experiments, each with two technical repeats. Plotted are the means of each group and the error bars represent the standard deviation of the mean. PBS and SAMTB lux infected larvae were compared to the naïve control using one-way ANOVA with Dunnett’s multiple comparison test. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.