Figure 9.
The effect of first-line and second-line antibiotics on the reduction of SAMTB lux bioluminescence within G. mellonella over a 96 h time course.
Larvae (n = 30/group) were infected with 2×107 CFU of SAMTB lux. At 1 h post-infection, larvae were treated with a single dose of INH (5 mg/kg), RIF (10 mg/kg), and BDQ (5.7 mg/kg) at the recommended clinical dosage relative to the larval body weigh; while PZA (250 mg/kg), ETH (150 mg/kg), MOX (67 mg/kg) were used at concentrations ten-folds higher than the recommended dosage. Control groups were injected larvae mock treated with PBS-T. At 0, 24, 48, 72, and 96 h post-infection, four larvae from each group were individually homogenized and the bioluminescence (RLU/ml) of the homogenates was measured to determine the relative drug efficacies. Data are pooled from three independent experiments. Plotted are the mean of each group and the error bars represent the standard deviation of the mean. Non-parametric Kruskal-Wallis test with Dunn’s multiple comparison was carried out against the PBS-T injected control for all treatment groups. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.