Figure 11.
The effect of RIF multiple dosing and RIF combination drug therapy on the reduction of SAMTB lux bioluminescence within G. mellonella.
Larvae (n = 30/group) were infected with 2×107 CFU of SAMTB lux. (a) At 1 h post-infection, larvae were treated with a single dose of RIF (10 mg/kg) and for dual dosing an additional dose was given 24 h post-infection. (b) At 1 h post-infection, larvae were treated with a single dose of RIF (10 mg/kg), ETH (15 mg/kg), MOX (6.7 mg/kg) or in combination as RIF/ETH or RIF/ETH/MOX. At 96 h post-infection, four larvae from each group were individually homogenized and the bioluminescence (RLU/ml) of the homogenate was measured to determine relative drug efficacy. The efficacy of dual RIF dosing was compared to single dosing using the Mann-Whitney test. As for combination drug therapy, the efficacy of each drug combination was compared to their respective single drug type efficacy using a non-parametric Kruskal-Wallis test with Dunn’s multiple comparison. For combination drug therapy, the color of * correlates to the color of the single drug type. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.