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. 2020 Jul 29;11(1):947–963. doi: 10.1080/21505594.2020.1793525

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© 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Cytotoxicity profile of the 30 investigated S. aureus ST398 isolates in HeLa cells. Hela cells were infected with bacteria at a MOI of 50:1. Upon 3 h incubation, the non-internalized bacteria were eliminated by washing the cells twice in fresh medium, and by bacterial killing with lysostaphin immediately after the last washing step. Subsequently, the HeLa cell viability was assayed by measuring the reduction of MTT. The results are presented as the percentage of MTT reduction relative to the uninfected control. The cytotoxicity of each S. aureus ST398 strain was assessed in three independent experiments. The two red lines mark the average MTT reduction upon HeLa cell infection with the investigated LA-ST398 or human-originated ST398 strains, respectively.