Key resources table.
Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Strain, strain background (species) | Escherichia coli (MRE600) | Gift of Arto Pulk, UC Berkeley | ATCC #29417, NCTC #8164 | Strain with low ribonuclease activity |
Other | 300 mesh R1.2/1.3 UltraAuFoil grids | Electron Microscopy Sciences | Q350AR13A | |
Software, algorithm | SerialEM | Schorb et al., 2019 | RRID:SCR_017293 | |
Software, algorithm | MotionCor2 | Zheng et al., 2017 | RRID:SCR_016499 | |
Software, algorithm | CTFFind4 | Rohou and Grigorieff, 2015 | RRID:SCR_016732 | |
Software, algorithm | RELION | Zivanov et al., 2018 | Version 3 and 3.1 RRID:SCR_016274 |
|
Software, algorithm | Cryosparc | Punjani et al., 2017 | Version 2 RRID:SCR_016501 |
|
Software, algorithm | Chimera | Pettersen et al., 2004 | RRID:SCR_004097 | |
Software, algorithm | PHENIX | Liebschner et al., 2019 | RRID:SCR_014224 | |
Software, algorithm | Coot | Casañal et al., 2020 | RRID:SCR_014222 |