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(A) Number of unique peptides and (B) protein groups identified from analysis of 100 HeLa cells, 20 HeLa cells, four single HeLa cells (SC-1, SC-2, SC-3, SC-4), and zero cells (blank) using the Orbitrap Eclipse without and with MBR identifications. (C) Venn diagram showing unique protein expression in four single HeLa cells. Note that SC-1–3 are average-sized HeLa cells (<20 μm diameter) and SC-4 is a large cell (~25 μm diameter).
