Figure 2.

Androgen-induced response on human fallopian tube static vs. dynamic conditions. (A) ELISA for vascular endothelial growth factor-A (VEGF-A) in conditioned media from hFTE treated with low testosterone vs. high, show a significant decrease in VEGF-A after 14 days. (B) Insulin-like growth factor-1 (IGF-1) secretion was unchanged in the presence of high testosterone. (C and D) Decreased oviduct-specific glicoprotein-1 (OVGP1) protein, in static culture, after exposure to high testosterone is compared to low testosterone using immunohistochemistry (IHC). Scale bar is 50 µm and all pictures have same magnification. (E and F) Decreased OVGP1 protein in the presence of high testosterone as compared to low using IHC in the microfluidic. (G) Reduction of OVGP1 expression confirmed by quantitative PCR (qPCR) in static tissues treated for 14 days. Experiments include four different patients and graph are represented as mean ± SEM. *P ≤ 0.05; **P ≤ 0.01; ****P ≤ 0.0001