Figure 4.

Exposure to PCOS-like (high) androgen concentrations alters expression of transcripts that encode for cilia in hFTE. (A) Heatmap of cilia mRNA expression in in PCOS-like (High) vs. control (Low). These data were generated using hFTE from three subjects treated with control or PCOS-like androgen levels in static culture for 14 days. Only significant genes with false discovery rate (FDR) adjusted P-value < 0.05 were included. (B) Schematic showing the role of FOXJ1 as master regulator of cilia genes and of LRRC6 and KIF5C regulation of intra-cilia transport that is critical for proper cilia function. (C) qPCR validation of FOXJ1, KIF5C, LRRC6 mRNA expression from three independent patient samples. *P ≤ 0.05; **P ≤ 0.01.