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. 2020 Aug 5;35(9):2086–2096. doi: 10.1093/humrep/deaa157

Figure 5.

Figure 5.

High androgen alters protein expression of FOXJ1, a regulator of cilia expression, in fluidic cultures. (A and B) IHC of acetylated tubulin (Ac. Tubulin), which marks cilia, in low vs. high testosterone-treated hFTE after 7 days treatment and 14 days. (C and D) IHC of FOXJ1 shows reduced expression and nuclear localization in high testosterone-treated hFTE after 7 days treatment. (E and F) IHC of acetylated tubulin (Ac. Tubulin), in low vs. high testosterone-treated hFTE after 14 days of treatment. (G and H) IHC of FOXJ1 shows reduced expression and nuclear localization in high testosterone-treated hFTE after 14 days treatment. Scale bar is 50 µm and magnification is the same for all pictures. Red squares show a 4× magnification of the image to visualize receptor localization.