Fig. 2.

CpGLP1 transcript expression analysis. a Dehydration and rehydration treatments. C. plantagineum plants were submitted to a dehydration-rehydration treatment. Samples were taken from untreated (100% relative water content; RWC), partially dehydrated (50% RWC), desiccated (2% RWC) and rehydrated (24 h) leaves. b Abscisic acid (ABA) treatment. Fully grown leaves were detached from plants and incubated for 2 h, 4 h and 8 h in a 100 µM ABA solution or in water as control. c Osmotic stress treatment. Fully grown leaves were detached from plants and incubated for 4 h, 24 h and 48 h in a 0.8 M mannitol solution or in water as control. Expression levels of the elongation factor 1α (EF1α) transcript were analyzed in all samples to monitor differences in input cDNA template. Expression of the dehydration-inducible CDeT11-24 transcript was measured as a control for the dehydration rehydration treatment (a). PCR products were obtained by either 30 (EF1α and CDeT11-24) or 32 (CpGLP1) amplification cycles