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Analysis of SOD activity. Total proteins were extracted from IPTG-induced BL21 cells harboring the CpGLP1 overexpression vector (+ CpGLP1) or the empty vector (− CpGLP1). Protein samples were separated on 12% SDS gels and gels were either stained with Coomassie blue staining total proteins, or blotted on a nitrocellulose membrane for immunodetection, or used for in-gel SOD enzymatic activity test
