Fig. 7.

The influence of a 5-week treatment with imipramine or agomelatine on the expression and activity of CYP3A in the liver from the chronic mild stress (CMS) model. a The mRNA expression level of CYP3A1/23 and CYP3A2. Results are expressed as the fold-change in relation to the housekeeping gene β-actin (n = 6–8), the outcome of two-way ANOVA is shown in Table 1; b CYP3A1/23 and CYP3A2 protein level (n = 6–8), the outcome of one-way ANOVA is shown in Table 2; c CYP3A activity measured as a rate of testosterone 6β-hydroxylation (n = 8–10), the outcome of two-way ANOVA is shown in Table 3. Duncan’s test: statistical significance is shown as *p < 0.05, **p < 0.01, ***p < 0.001 compared to the nonstressed control; ⁺p < 0.05, ⁺⁺p < 0.01 compared to the stressed control; ^#p < 0.05 compared to the nonstressed agomelatine-treated rats. The representative CYP3A1 and CYP3A2 protein bands of the Western immunoblot analysis are shown in Fig. 8. CON control, IMI imipramine, AGM agomelatine