Table 2.
siRNA duplex validation results in the secondary deconvolution screen.
| Screen Output Parameters | siRNA Duplex Validation | Phenotype Confirmed | ||||
|---|---|---|---|---|---|---|
| 0/4 | 1/4 | 2/4 | 3/4 | 4/4 | ||
| Control cell count (T48) bin | 83 | 149 | 104 | 52 | 12 | 168 |
| Percentage | 21% | 37% | 26% | 13% | 3% | 42% |
| Drug cell count (T48) bin | 61 | 133 | 123 | 65 | 18 | 206 |
| Percentage | 15.2% | 33.2% | 30.8% | 16.2% | 4.5% | 51.5% |
| Control viability (DeltaT) bin | 89 | 112 | 81 | 79 | 39 | 199 |
| Percentage | 22.2% | 28% | 20.2% | 19.8% | 9.8% | 49.8% |
| Drug viability (DeltaT) bin | 66 | 94 | 85 | 104 | 51 | 240 |
| Percentage | 16% | 24% | 21% | 26% | 13% | 60% |
| Control lactate/cell bin | 137 | 140 | 78 | 30 | 15 | 123 |
| Percentage | 34.2% | 35% | 19.5% | 7.5% | 3.8% | 30.8% |
| Drug lactate/Control lactate per cell ratio < 0.55 | 183 | 4 | 121 | 71 | 21 | 213 |
| Percentage | 45% | 1% | 30.25% | 17.75% | 5.25% | 53.25% |