Fig. 2.

IUE-mediated expression of Yap1-S112A and Tead2-VP16 enhanced stem-like characteristics in telencephalic and diencephalic NPCs, as determined by protocols shown in a. Anti-Yap1 immunoreactivity increased in Yap-S112A–electroporated VZ cells (b). The proportion of total GFP⁺ cells that were also positive for Pax6 was greater in Yap1-S112A or Tead2-VP16 IUE cases in both telencephalon and diencephalon (c). The proportion of GFP⁺BrdU⁺ cells that were negative for p27 or positive for Pax6 was greater in Yap1-S112A– or Tead-VP16–electroporated diencephalic NPCs (d). Scale bars 10 µm in b, 5 µm in c and d