Fig. 5.

Effect of metformin on apoptosis induction in malignant plasma cells in normoxic and hypoxic conditions. L363 and RPMI 8226 cells were cultured for 48 h in hypoxic and/or normoxic conditions in the presence or absence of metformin (5 mM) and subsequently stained by Annexin V/PI followed by FACS analysis. The representative experiment of three independent ones is presented. The living cells were defined as Annexin V negative/PI negative, early apoptotic cells were defined as Annexin V positive/PI negative and late apoptotic cells were defined as Annexin V positive/PI positive. The % of early, late apoptotic cells and living cells in particular condition is given as the % of gated cells. The mean ± SEM of three independent experiments is shown. According to multiway ANOVA, there were no differences between % of living, early apoptotic and late apoptotic cells in different conditions