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. 2020 Sep 29;11:564627. doi: 10.3389/fimmu.2020.564627

Figure 2.

Figure 2

Cytometric bead array analysis of antigen-specific cytokine responses induced by immunization with chimeric VLPs or capsomeres. BALB/c mice (n = 5/group) were immunized with either a homologous (no DNA prime) or heterologous (DNA prime-boost) regimen as described in the legend to Figure 1. Seven days after the third immunization splenocytes were harvested and single cell suspensions stimulated with either the CD8+ (280−288), or CD4+ (58−79) T cell peptides or with pooled peptides including both peptides plus the B cell repeat peptide and incubated for 72 h at 37°C and 5% CO2. Culture supernatant was assayed using a cytometric bead array to quantify IFN-γ, TNF, IL-2, and IL-6 cytokine levels. Data are displayed as mean pg/ml + SEM for each cytokine with statistical comparisons made to the PBS control group with significance determined using one-way ANOVA followed by Bonferroni's post-test. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.