Skip to main content
. 2020 Sep 29;11:576903. doi: 10.3389/fimmu.2020.576903

Figure 1.

Figure 1

Hsa_circ_0060450 is upregulated in the PBMCs of T1DM patients. (A) Heatmap of circRNAs with significantly altered expression in the PBMCs of 3 normal controls and 3 T1DM patients. (B) Volcano plot of circRNAs with significantly altered expression in the PBMCs of 3 normal controls and 3 T1DM patients. (C) The expression of hsa_circ_0060450 in PBMCs of 20 children with T1DM and 20 healthy controls, detected by RT-qPCR. (D) Receiver operating curve (ROC) analysis of hsa_circ_0060450 levels in the study population. (E) The genomic loci of the MYBL2 and hsa_circ_0060450. The expression of hsa_circ_0060450 was detected by RT-PCR and validated by Sanger sequencing. (F) RT-qPCR analyses of β-actin, MYBL2, and hsa_circ_0060450 after treatment with RNase R in THP1-derived macrophages. (G) RT-qPCR analyses of MYBL2 and hsa_circ_0060450 after treatment with actinomycin D at the indicated time points in THP1-derived macrophages. (H) The expressions of hsa_circ_0060450 in T cells, B cells and monocytes of human PBMCs, detected by RT-qPCR. (I) The relative distribution of hsa_circ_0060450 in the nucleus and cytoplasm in THP1-derived macrophages. circ_0450 is the abbreviation of hsa_circ_0060450. ActD, actinomycin D. Data represent means ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001. ns, not significance.