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. 2020 Sep 29;11:576903. doi: 10.3389/fimmu.2020.576903

Figure 4.

Figure 4

miR-199a-5p promotes IFN-I-induced inflammation by targeting SHP2. (A) RT-qPCR analyses of five predicted miR-199a-5p target genes after treatment with miR-199a-5p mimics. (B) SHP2 protein assessment at 0, 15, and 30 min of IFN-I stimulation after treatment with miR-199a-5p mimics. (C) Three miR-199a-5p binding sites within SHP2 3′ UTR predicted using the starBase database. (D) Luciferase activities of 293T cells co-transfected with blank psicheck2 vector or psicheck2 recombinant vector containing SHP2 3′ UTR fragment and miR-199a-5p mimics or NC. NC, negative control. SHP2-v, psicheck2 recombinant vector containing SHP2 3′ UTR fragment. Data represent means ± SEM. *p < 0.05, **p < 0.01. ns, not significance.